CRISPR technologies for precise epigenome editing

Nat Cell Biol. 2021 Jan;23(1):11-22. doi: 10.1038/s41556-020-00620-7. Epub 2021 Jan 8.

Abstract

The epigenome involves a complex set of cellular processes governing genomic activity. Dissecting this complexity necessitates the development of tools capable of specifically manipulating these processes. The repurposing of prokaryotic CRISPR systems has allowed for the development of diverse technologies for epigenome engineering. Here, we review the state of currently achievable epigenetic manipulations along with corresponding applications. With future optimization, CRISPR-based epigenomic editing stands as a set of powerful tools for understanding and controlling biological function.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • CRISPR-Cas Systems*
  • Epigenesis, Genetic*
  • Epigenome*
  • Gene Editing*
  • Gene Expression Regulation*
  • Humans