Oxygen Consumption Evaluation: An Important Indicator of Metabolic State, Cellular Function, and Cell Fate Along Neural Deregulation

Methods Mol Biol. 2021;2240:207-230. doi: 10.1007/978-1-0716-1091-6_15.

Abstract

Depletion of oxygen (O2) levels and reduction in the ATP synthesis (or even its complete blockage) are important characteristics of mitochondrial dysfunction; features that are often correlated with neurodegeneration. The measurement of oxygen consumption rate (OCR) is thus essential to evaluate cellular metabolism, survival, and neuroprotective strategies. In the present chapter, we describe the oxygen consumption assay using a Clark-type oxygen electrode in different types of samples named cells suspension (from primary and established cell culture), brain slices (ex vivo), and fresh brain tissues. In addition, we demonstrate herein how the program Oxygraph can be used in order to analyze the data and different approaches to normalize it.

Keywords: Brain slice; Cell suspension; Cellular function; Metabolism; Mitochondria; Neural deregulation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism*
  • Animals
  • Biological Assay*
  • Brain / drug effects
  • Brain / metabolism*
  • Cell Line
  • Humans
  • In Vitro Techniques
  • Mitochondria / drug effects
  • Mitochondria / metabolism*
  • Neurons / drug effects
  • Neurons / metabolism*
  • Oxidative Phosphorylation* / drug effects
  • Oxygen Consumption* / drug effects
  • Primary Cell Culture
  • Rats
  • Time Factors

Substances

  • Adenosine Triphosphate