Antifungal activity of nanoemulsion from Cleome viscosa essential oil against food-borne pathogenic Candida albicans

Rajapandiyan Krishnamoorthy; Mustafa A Gassem; Jegan Athinarayanan; Vaiyapuri Subbarayan Periyasamy; Saradh Prasad; Ali A Alshatwi

doi:10.1016/j.sjbs.2020.10.001

Antifungal activity of nanoemulsion from Cleome viscosa essential oil against food-borne pathogenic Candida albicans

Saudi J Biol Sci. 2021 Jan;28(1):286-293. doi: 10.1016/j.sjbs.2020.10.001. Epub 2020 Oct 15.

Authors

Rajapandiyan Krishnamoorthy¹, Mustafa A Gassem¹, Jegan Athinarayanan¹, Vaiyapuri Subbarayan Periyasamy¹, Saradh Prasad², Ali A Alshatwi¹

Affiliations

¹ Department of Food Science and Nutrition, College of Food and Agriculture Sciences, King Saud University, Post box 2460, Riyadh 11541, Saudi Arabia.
² Research Chair on Laser Diagnosis of Cancers, Department of Physics, College of Science, King Saud University, 11451 Riyadh, Saudi Arabia.

Abstract

Pathogenic and spoilage fungi cause enormous challenges to food related fatal infections. Plant essential oil based classical emulsions can functions as antifungal agents. To investigate the antifungal spectrum, that is the scope of the nanoemulsion composed of Cleome viscosa essential oil and Triton-x-100 fabricated by ultrasonication method. Minimum inhibitory and fungicidal concentration of essential oil nanoemulsion (EONE) was tested against food borne pathogenic C. albicans. The MIC and MFC values ranged from 16.5 to 33 µl/ml with significant reduction on biofilm of C. albicans isolates. The alteration of molecular fingerprints was confirmed by Fourier transformed infrared spectroscopy and subsequent reduction of chitin levels in cell walls was noted by spectroscopic analysis. The EONE and their bioactive compounds cause collateral damage on C. albicans cells.

Keywords: Anti-fungal activity Anti biofilm activity; Food borne pathogen; Fungal cell wall inhibitor; Nanoemulsion.