Effects of cannabidiol (CBD) on the inhibition of melanoma cells in vitro

J Immunoassay Immunochem. 2021 May 4;42(3):285-291. doi: 10.1080/15321819.2020.1862863. Epub 2021 Jan 11.


Cannabidiol (CBD) is one of the most popular emerging plant extracts that is being investigated for its wide range of potential health benefits. This experiment tests how B16 mice melanoma cells, are affected by four different concentrations (0.2 mg/mL, 0.04 mg/mL, 0.008 mg/mL and 0.0016 mg/mL) of 99% CBD oil. The results of this experiment demonstrate that CBD significantly inhibited melanoma cell growth in-vitro at 0.2 mg/mL and 0.04 mg/mL. This shows that CBD has the potential to inhibit melanoma cell growth in vertebrates, namely mice.

Keywords: CBD; Cannabidiol; in vitro; melanoma; mice; spleen T cells; spleen cells.

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Cannabidiol / chemistry
  • Cannabidiol / pharmacology*
  • Cannabis / chemistry
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor
  • Melanoma / drug therapy*
  • Melanoma / pathology
  • Mice
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*


  • Antineoplastic Agents, Phytogenic
  • Plant Extracts
  • Cannabidiol