Abstract
Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplification (LAMP) method for the detection of cdtA, cdtB, and cdtC genes was established which showed high specificity and strong sensitivity. The LAMP assay showed a detection threshold was 3.13 pg/μl within 40 min.
Keywords:
Cytolethal distending toxins; Diarrhea; LAMP; Providencia alcalifaciens.
Copyright © 2021 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Toxins / isolation & purification*
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Enterobacteriaceae Infections* / diagnosis
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Enterobacteriaceae Infections* / microbiology
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Humans
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Molecular Diagnostic Techniques / methods*
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Nucleic Acid Amplification Techniques / methods*
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Providencia* / isolation & purification
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Providencia* / metabolism
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Virulence Factors / isolation & purification*
Substances
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Bacterial Toxins
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Virulence Factors
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cytolethal distending toxin
Supplementary concepts
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LAMP assay
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Providencia alcalifaciens