Sensitive and rapid detection of cytolethal distending toxin encoding genes in Providencia alcalifaciens

J Microbiol Methods. 2021 Feb:181:106143. doi: 10.1016/j.mimet.2021.106143. Epub 2021 Jan 9.

Abstract

Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplification (LAMP) method for the detection of cdtA, cdtB, and cdtC genes was established which showed high specificity and strong sensitivity. The LAMP assay showed a detection threshold was 3.13 pg/μl within 40 min.

Keywords: Cytolethal distending toxins; Diarrhea; LAMP; Providencia alcalifaciens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins / isolation & purification*
  • Enterobacteriaceae Infections* / diagnosis
  • Enterobacteriaceae Infections* / microbiology
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Nucleic Acid Amplification Techniques / methods*
  • Providencia* / isolation & purification
  • Providencia* / metabolism
  • Virulence Factors / isolation & purification*

Substances

  • Bacterial Toxins
  • Virulence Factors
  • cytolethal distending toxin

Supplementary concepts

  • LAMP assay
  • Providencia alcalifaciens