Topoisomerase IV tracks behind the replication fork and the SeqA complex during DNA replication in Escherichia coli

Sci Rep. 2021 Jan 12;11(1):474. doi: 10.1038/s41598-020-80043-4.


Topoisomerase IV (TopoIV) is a vital bacterial enzyme which disentangles newly replicated DNA and enables segregation of daughter chromosomes. In bacteria, DNA replication and segregation are concurrent processes. This means that TopoIV must continually remove inter-DNA linkages during replication. There exists a short time lag of about 10-20 min between replication and segregation in which the daughter chromosomes are intertwined. Exactly where TopoIV binds during the cell cycle has been the subject of much debate. We show here that TopoIV localizes to the origin proximal side of the fork trailing protein SeqA and follows the movement pattern of the replication machinery in the cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / metabolism
  • Chromosomes, Bacterial / metabolism*
  • DNA Replication / physiology*
  • DNA Topoisomerase IV / antagonists & inhibitors
  • DNA Topoisomerase IV / metabolism*
  • DNA-Binding Proteins / metabolism
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Escherichia coli Proteins / metabolism
  • Topoisomerase II Inhibitors / pharmacology


  • Bacterial Outer Membrane Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • SeqA protein, E coli
  • Topoisomerase II Inhibitors
  • DNA Topoisomerase IV