T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood-Based Assays Ready to Challenge ELISPOT?

Open Forum Infect Dis. 2020 Nov 8;8(1):ofaa547. doi: 10.1093/ofid/ofaa547. eCollection 2021 Jan.


We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood-based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.

Keywords: ELISPOT; T cells; aspergillosis; biomarker; cytokines; cytomegalovirus; flow cytometry; immunoassay.