We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood-based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.
Keywords: ELISPOT; T cells; aspergillosis; biomarker; cytokines; cytomegalovirus; flow cytometry; immunoassay.
© The Author(s) 2020. Published by Oxford University Press on behalf of Infectious Diseases Society of America.