Nitrite accumulation usually occurred when domesticating the idle aerobic activated sludge. A sequencing batch reactor (SBR) was used to investigate whether the short-cut nitrification sludge could be cultivated using the idle sludge as inoculated sludge. The results showed that the nitrification process consisted of three stages. In the first stage, the activity of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) were very low with almost no nitrification performance. In the second stage, the activity of AOB started to recover with the effluent NH4+-N gradually decreased to 0.29 mg L-1, while NOB was alternately inhibited by free ammonia (FA), free nitrous acid (FNA), and nitrite. The effluent NOx--N was mainly NO2--N with an average nitrite accumulation ratio of 74.00%. In the third stage, the nitrification altered from short-cut nitrification to complete nitrification, and the nitrification kinetics of AOB and NOB were both well-fitted to the Monod equation (R2 > 0.92). The variations of effluent pH and ORP between cycles could indicate the recovery stage of the nitrifying ability. Through monitoring the curves of effluent pH and ORP, when the domestication process is between the pH peak and ORP plateau, the short-cut nitrification sludge could be cultivated. This study revealed the mechanism of nitrite accumulation during the domestication of long-term idle aerobic activated sludge, and established a control strategy to accelerate the domestication.
Keywords: Activity recovery; Idle sludge; Nitrification kinetics; Real-time control; Short-cut nitrification; Simultaneous nitrification and denitrification.