Cultured cardiac fibroblasts and myofibroblasts express Sushi Containing Domain 2 and assemble a unique fibronectin rich matrix

Exp Cell Res. 2021 Feb 15;399(2):112489. doi: 10.1016/j.yexcr.2021.112489. Epub 2021 Jan 14.


Cardiac fibroblasts and myofibroblasts assemble and maintain extracellular matrix during normal development and following injury. Culture expansion of these cells yield a bioengineered matrix that could lead to intriguing therapeutic opportunities. For example, we reported that cultured rat cardiac fibroblasts form a matrix that can be used to delivery therapeutic stem cells. Furthermore, we reported that matrix derived from cultured human cardiac fibroblasts/myofibroblasts converted monocytes into macrophages that express interesting anti-inflammatory and pro-angiogenic properties. Expanding these matrix investigations require characterization of the source cells for quality control. In these efforts, we observed and herein report that Sushi Containing Domain 2 (SUSD2) is a novel and consistent marker for cultured human cardiac fibroblast and myofibroblasts.

Keywords: Biomaterial; Cardiac fibroblast; Cell-derived matrix; Dermal fibroblast; Fibronectin; Matrix; SUSD2; Sushi containing domain 2.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers / metabolism
  • Cells, Cultured
  • Extracellular Matrix / metabolism*
  • Extracellular Matrix / physiology
  • Female
  • Fibroblasts / metabolism
  • Fibronectins / metabolism
  • Humans
  • Male
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Myocardium / cytology
  • Myocardium / metabolism*
  • Myofibroblasts / metabolism


  • Biomarkers
  • Fibronectins
  • Membrane Glycoproteins
  • SUSD2 protein, human