GABA-like immunoreactivity in cholinergic amacrine cells of the rabbit retina

Brain Res. 1988 Jan 12;438(1-2):369-73. doi: 10.1016/0006-8993(88)91366-2.

Abstract

In the ganglion cell layer of the rabbit retina, the inhibitory transmitter gamma-aminobutyric acid (GABA) and its analogues are accumulated by neurons that appear to match in size and number the population of displaced amacrine cells that synthesize the excitatory transmitter acetylcholine. In this double-label study, we have established directly that the cholinergic amacrine cells, selectively stained with diamidino-phenylindole, are strongly immunoreactive with GABA antisera. The coexistence of two classical transmitters, one excitatory and the other inhibitory, in this defined neuronal population, suggests that stimulation of the cholinergic amacrines may give rise to complex responses in their target neurons.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholine / analysis*
  • Animals
  • Cholinergic Fibers / analysis
  • Cholinergic Fibers / metabolism*
  • Fluorescent Dyes
  • Immunohistochemistry
  • Indoles
  • Rabbits
  • Retina / metabolism*
  • Retinal Ganglion Cells / analysis
  • Retinal Ganglion Cells / metabolism*
  • gamma-Aminobutyric Acid / analysis*

Substances

  • Fluorescent Dyes
  • Indoles
  • DAPI
  • gamma-Aminobutyric Acid
  • Acetylcholine