Complete mitochondrial genome of the gray reef shark, Carcharhinus amblyrhynchos (Carcharhiniformes: Carcharhinidae)

Nicholas Dunn; Shaili Johri; David Curnick; Chris Carbone; Elizabeth A Dinsdale; Taylor K Chapple; Barbara A Block; Vincent Savolainen

doi:10.1080/23802359.2020.1765208

Complete mitochondrial genome of the gray reef shark, Carcharhinus amblyrhynchos (Carcharhiniformes: Carcharhinidae)

Mitochondrial DNA B Resour. 2020 May 14;5(3):2080-2082. doi: 10.1080/23802359.2020.1765208.

Authors

Nicholas Dunn^{1

2}, Shaili Johri^{3

4}, David Curnick¹, Chris Carbone¹, Elizabeth A Dinsdale⁴, Taylor K Chapple⁵, Barbara A Block³, Vincent Savolainen²

Affiliations

¹ Institute of Zoology, Zoological Society of London, London, UK.
² Department of Life Sciences, Imperial College London, Ascot, UK.
³ Hopkins Marine Station, Stanford University, Pacific Grove, CA, USA.
⁴ Department of Biology, San Diego State University, San Diego, CA, USA.
⁵ Coastal Oregon Marine Experiment Station, Oregon State University, Newport, OR, USA.

Abstract

We report the first mitochondrial genome sequences for the gray reef shark, Carcharhinus amblyrhynchos. Two specimens from the British Indian Ocean Territory were sequenced independently using two different next generation sequencing methods, namely short read sequencing on the Illumina HiSeq and long read sequencing on the Oxford Nanopore Technologies' MinION sequencer. The two sequences are 99.9% identical and are 16,705 base pairs (bp) and 16,706 bp in length. The mitogenome contains 22 tRNA genes, two rRNA genes, 13 protein-coding genes and two non-coding regions; the control region and the origin of light-strand replication (OL).

Keywords: British Indian Ocean Territory; Carcharhinus amblyrhynchos; Shark; mitochondrial genome.

Grant support

We thank the Bertarelli Foundation and the UK Natural Environment Research Council for funding, Robert Edwards and Adrian Cantu for computing support, Danny Coffey, Tim White, Nathan Truelove, Robert Schallert, and David Jacoby for collecting samples, Theodore Reimer for logistics and CD-genomics for assistance with Illumina sequencing.