Next-generation sequencing yields the complete mitochondrial genome of Microhyla beilunensis (Anura: Microhylidae)

Lei Feng; Wei-Cheng Zheng; Zhi-Qiang Chen; Jun-Jie Zhong; Guo-Hua Ding

doi:10.1080/23802359.2020.1827068

Next-generation sequencing yields the complete mitochondrial genome of Microhyla beilunensis (Anura: Microhylidae)

Mitochondrial DNA B Resour. 2020 Oct 9;5(3):3529-3530. doi: 10.1080/23802359.2020.1827068.

Authors

Lei Feng¹, Wei-Cheng Zheng², Zhi-Qiang Chen¹, Jun-Jie Zhong¹, Guo-Hua Ding¹

Affiliations

¹ Laboratory of Amphibian Diversity Investigation, College of Ecology, Lishui University, Lishui, Zhejiang, China.
² Administration Center of Jiulongshan National Nature Reserve, Suichang, Zhejiang, China.

Abstract

The complete mitochondrial genome (mtDNA) of Microhyla beilunensis (Anura: Microhylidae) was sequenced and annotated. The length of mtDNA sequences of M. beilunensis was 16,721 bp, and encoded 13 protein-coding genes (PCGs), 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and a control region. The overall nucleotide composition of this genome was 29.1% A, 24.5% C, 14.5% G, 31.9% T, with a total A + T content of 61%. Phylogenetic analysis using Bayesian Inference (BI) method revealed that M. beilunensis was closely related with other 8 species from the genus Microhyla. The mtDNA dataset could be utilized for studying the molecular ecology and population genetics of Microhylid frogs.

Keywords: Illumina; Microhyla beilunensis; mitochondrial genome; phylogenetic analysis.

Grants and funding

This work was supported by Zhejiang Provincial Natural Science Foundation of China [LQ16C040001], National Science Foundation of China [31500308] and A Cooperative Project between Suichang country and University [HZ2019-07].