Calcium imaging of adult-born neurons in freely moving mice

Alvaro Carrier-Ruiz; Yuki Sugaya; Deependra Kumar; Pablo Vergara; Iyo Koyanagi; Sakthivel Srinivasan; Toshie Naoi; Masanobu Kano; Masanori Sakaguchi

doi:10.1016/j.xpro.2020.100238

Calcium imaging of adult-born neurons in freely moving mice

STAR Protoc. 2020 Dec 31;2(1):100238. doi: 10.1016/j.xpro.2020.100238. eCollection 2021 Mar 19.

Authors

Alvaro Carrier-Ruiz^{1

2

3}, Yuki Sugaya^{1

2}, Deependra Kumar⁴, Pablo Vergara⁴, Iyo Koyanagi⁴, Sakthivel Srinivasan⁴, Toshie Naoi⁴, Masanobu Kano^{1

2}, Masanori Sakaguchi⁴

Affiliations

¹ Department of Neurophysiology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-0033, Japan.
² International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study (UTIAS), Tokyo 113-0033, Japan.
³ Department of Neuroscience, Karolinska Institute, Stockholm 17165, Sweden.
⁴ International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, Tsukuba, Ibaraki 305-0006, Japan.

Abstract

Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca²⁺ imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).

Keywords: Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium Signaling*
Dentate Gyrus / metabolism*
Hippocampus / metabolism*
Intravital Microscopy*
Mice
Microscopy, Fluorescence
Neurons / metabolism*