Regulation of interferon gene expression: mechanism of action of the If-1 locus

J Virol. 1988 Apr;62(4):1125-31. doi: 10.1128/JVI.62.4.1125-1131.1988.


We have examined the mechanism of action of the If-1 interferon (IFN) regulatory locus. This locus controls the level of circulating IFN produced in inbred mice in response to intravenous injection of Newcastle disease virus. Mice carrying the If-1h (high) allele show circulating IFN levels 10- to 15-fold higher than those carrying the If-1l (low) allele. In this report we show that induced splenocytes from If-1h and If-1l mice produce IFN at levels which are in the same proportions as those found in the circulation. Higher levels of IFN-specific mRNA were observed in splenocyte populations from If-1h animals. This was due to increased transcription of IFN genes. At the same time, the high- and low-producing populations showed no significant difference in the number of IFN mRNA-containing cells. We conclude that the effect of If-1 in the spleen is to control the levels of transcription of the IFN genes in individual induced splenocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Gene Expression Regulation*
  • Genes, Regulator*
  • Interferon Type I / biosynthesis
  • Interferon Type I / genetics*
  • Kinetics
  • Male
  • Mice
  • Mice, Inbred Strains
  • Newcastle disease virus / physiology
  • Nucleic Acid Hybridization
  • RNA, Viral / analysis
  • Spleen / cytology
  • Spleen / immunology
  • Transcription, Genetic


  • Interferon Type I
  • RNA, Viral