The spindle pole-body localization of activated cytoplasmic dynein is cell cycle-dependent in Aspergillus nidulans

Fungal Genet Biol. 2021 Mar;148:103519. doi: 10.1016/j.fgb.2021.103519. Epub 2021 Jan 17.

Abstract

Cytoplasmic dynein is a minus end-directed microtubule motor that can be activated by cargo adapters. In Aspergillus nidulans, overexpression of ΔC-HookA, the early endosomal adapter HookA missing its cargo-binding site, causes activated dynein to accumulate at septa and spindle pole bodies (SPBs) where the microtubule-organizing centers are located. Intriguingly, only some interphase nuclei show SPB signals of dynein. Here we present data demonstrating that localization of the activated dynein at SPBs is cell cycle-dependent: SPB dynein signals are seen to associate with nuclei at early G1 but disappear at about the G1-S boundary.

Keywords: Aspergillus nidulans; Dynein; Spindle-pole body.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Aspergillus nidulans / genetics
  • Aspergillus nidulans / metabolism*
  • Binding Sites
  • Cell Cycle*
  • Cytoplasmic Dyneins / genetics
  • Cytoplasmic Dyneins / metabolism*
  • Protein Binding
  • Protein Transport
  • Spindle Poles / metabolism*

Substances

  • Cytoplasmic Dyneins