Dynamic Contrast Enhanced (DCE) MRI-Derived Renal Perfusion and Filtration: Experimental Protocol

Methods Mol Biol. 2021:2216:429-441. doi: 10.1007/978-1-0716-0978-1_25.

Abstract

Dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) can provide a noninvasive way for assessing renal functional information following the administration of a small molecular weight gadolinium-based contrast agent. This method may be useful for investigating renal perfusion and glomerular filtration rates of rodents in vivo under various experimental (patho)physiological conditions. Here we describe a step-by-step protocol for DCE-MRI studies in small animals providing practical notes on acquisition parameters, sequences, T1 mapping approaches and procedures.This chapters is based upon work from the COST Action PARENCHIMA, a community-driven network funded by the European Cooperation in Science and Technology (COST) program of the European Union, which aims to improve the reproducibility and standardization of renal MRI biomarkers. This experimental protocol chapter is complemented by two separate chapters describing the basic concept and data analysis.

Keywords: Contrast agent; Dynamic contrast-enhanced (DCE); Kidney; Magnetic resonance imaging (MRI); Mice; Rats; T1 map.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Animals
  • Contrast Media*
  • Glomerular Filtration Rate
  • Image Enhancement / methods*
  • Image Processing, Computer-Assisted / methods*
  • Kidney / physiology*
  • Magnetic Resonance Imaging / methods*
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Nude
  • Monitoring, Physiologic / methods*
  • Perfusion
  • Software

Substances

  • Contrast Media