Protocol for isolation of spermatids from mouse testes

STAR Protoc. 2021 Jan 8;2(1):100254. doi: 10.1016/j.xpro.2020.100254. eCollection 2021 Mar 19.


Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a simple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

Keywords: Cell Biology; Cell isolation; Model Organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Separation*
  • Male
  • Mice
  • Spermatids / cytology*
  • Spermatids / metabolism
  • Spermatogenesis
  • Testis / cytology*
  • Testis / metabolism