An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells

STAR Protoc. 2021 Jan 12;2(1):100264. doi: 10.1016/j.xpro.2020.100264. eCollection 2021 Mar 19.


In T cell-based cancer immunotherapy, tumor antigen (Ag)-specific CD8+ cytotoxic T lymphocytes (CTLs) can specifically target tumor Ags on malignant cells. This promising approach drove us to adopt this strategy of T cell transfer (ACT)-based immunotherapy for chronic viral infections. Here, we describe the generation of hepatitis B virus (HBV) Ag-specific CTLs from induced pluripotent stem cells (iPSCs), i.e., iPSC-CTLs. Ag-specific iPSC-CTLs can target HBV Ag+ cells and infiltrate into the liver to suppress HBV replication in a murine model. For complete details on the use and execution of this protocol, please refer to Haque et al. (2020).

Keywords: Immunology; Stem Cells.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Hepatitis B / immunology
  • Hepatitis B / therapy
  • Hepatitis B Antigens / immunology*
  • Hepatitis B virus / immunology*
  • Humans
  • Immunotherapy, Adoptive
  • Induced Pluripotent Stem Cells / immunology*
  • Mice
  • T-Lymphocytes, Cytotoxic / immunology*


  • Hepatitis B Antigens