Metabolic mediators determine the association of antinuclear antibody subtypes with specific clinical symptoms in systemic sclerosis

Adv Med Sci. 2021 Mar;66(1):119-127. doi: 10.1016/j.advms.2020.12.007. Epub 2021 Jan 22.

Abstract

Purpose: The aim of this study was to investigate the possible link between different types of systemic sclerosis-specific antinuclear antibodies, adipokines and endothelial molecules which were recently found to have a pathogenic significance in systemic sclerosis.

Materials/methods: Serum concentration of adiponectin, resistin, leptin, endothelin-1, fractalkine and galectin-3 were determined in the sera of patients with systemic sclerosis (n ​= ​100) and healthy controls (n ​= ​20) using ELISA.

Results: The following associations between antinuclear antibodies and increased serum concentrations were identified: anticentromere antibodies with endothelin-1 (p ​< ​0.0001; mean level in patients 2.21 vs control group 1.31 ​pg/ml), anti-topoisomerase I antibodies with fractalkine (p ​< ​0.0001; 3.68 vs 1.68 ​ng/ml) and galectin-3 (p ​= ​0.0010, 6.39 vs 3.26 ​ng/ml). Anti-RNA polymerase III antibodies were associated with increased resistin (p ​< ​0.0001; 15.13 vs 8.54 ​ng/ml) and decreased adiponectin (p ​< ​0.0001; 2894 vs 8847 ​ng/ml).

Conclusion: In systemic sclerosis metabolic and vascular factors may serve as mediators between immunological abnormalities and non-immune driven clinical symptoms.

Keywords: Adipokines; Adipose tissue; Antinuclear antibodies; Endothelium; Systemic sclerosis.

MeSH terms

  • Adipokines / blood
  • Adipokines / immunology
  • Adiponectin / blood
  • Adiponectin / immunology
  • Antibodies, Antinuclear / blood
  • Antibodies, Antinuclear / immunology*
  • Biomarkers / blood*
  • Blood Proteins / immunology
  • Case-Control Studies
  • Chemokine CX3CL1 / blood
  • Chemokine CX3CL1 / immunology
  • Endothelin-1 / blood
  • Endothelin-1 / immunology
  • Female
  • Follow-Up Studies
  • Galectins / blood
  • Galectins / immunology
  • Humans
  • Leptin / blood
  • Leptin / immunology
  • Male
  • Middle Aged
  • Prognosis
  • Resistin / blood
  • Resistin / immunology
  • Scleroderma, Systemic / blood
  • Scleroderma, Systemic / immunology
  • Scleroderma, Systemic / pathology*

Substances

  • Adipokines
  • Adiponectin
  • Antibodies, Antinuclear
  • Biomarkers
  • Blood Proteins
  • Chemokine CX3CL1
  • Endothelin-1
  • Galectins
  • LGALS3 protein, human
  • Leptin
  • RETN protein, human
  • Resistin