Protective Effects of Gynostemma pentaphyllum (var. Ginpent) against Lipopolysaccharide-Induced Inflammation and Motor Alteration in Mice

Molecules. 2021 Jan 22;26(3):570. doi: 10.3390/molecules26030570.


Gynostemma pentaphyllum (var. Ginpent) (GP) is a variety of Cucurbit with anti-inflammatory and antioxidant effects in patients. In this manuscript, the main components present in the dry extract of GP have been identified using Ultra High Performance Liquid Chromatography quadrupole-time-of-flight mass spectrometry (UHPLC/Q-TOF-MS). In addition, the anti-inflammatory action of GP was evaluated in animal models with acute peripheral inflammation and motor alteration induced by lipopolysaccharide. The results showed that GP dry extract is rich in secondary metabolites with potential antioxidant and anti-inflammatory properties. We found that the treatment with GP induced a recovery of motor function measured with the rotarod test and pole test, and a reduction in inflammatory cytokines such as interleukin-1β and interleukin-6 measured with the ELISA test. The data collected in this study on the effects of GP in in vivo models may help integrate the therapeutic strategies of inflammatory-based disorders.

Keywords: ELISA; Gynostemma pentaphyllum; anti-inflammatory; motility; supplement.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Antioxidants / pharmacology
  • Cytokines / metabolism
  • Gynostemma / chemistry*
  • Humans
  • Inflammation / chemically induced
  • Inflammation / metabolism
  • Inflammation / prevention & control*
  • Lipopolysaccharides / antagonists & inhibitors
  • Lipopolysaccharides / toxicity
  • Male
  • Mice
  • Mice, 129 Strain
  • Mice, Inbred C57BL
  • Motor Activity / drug effects*
  • Phytosterols / analysis
  • Phytotherapy
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology
  • Polyphenols / analysis
  • Saponins / analysis


  • Anti-Inflammatory Agents
  • Antioxidants
  • Cytokines
  • Lipopolysaccharides
  • Phytosterols
  • Plant Extracts
  • Polyphenols
  • Saponins