Mitigating effects of chitosan coating on postharvest senescence and energy depletion of harvested pummelo fruit response to granulation stress

Abstract

The effect of chitosan coating exposure on juice sac granulation and energy metabolism in harvested pummelo fruit was investigated. Pummelo fruits were exposed to 1.5% chitosan coating, and then stored at 20 ± 2 °C for about 150 days. Postharvest chitosan coating treatment apparently alleviated the development of juice sac granulation as well as the increases in weight loss, pulp firmness, cell membrane permeability and cellulose content. The levels of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and energy charge (EC) in the chitosan-coated fruit showed significantly higher levels than those of the respective controls. Meanwhile, the enzymses actively engaged in energy metabolism such as H⁺-ATPase, Ca²⁺-ATPase, Mg²⁺-ATPase, cytochrome C oxidase (CCO), succinate dehydrogenase (SDH) and malate dehydrogenase (MDH) were markedly maintained by chitosan coating. Besides, notably high contents of acetyl-CoA, cis-aconitate, succinate, fumarate and oxaloacetate were observed in the chitosan-coated fruit. The results highlighted that chitosan coating could delay postharvest senescence of pummelo fruit by reducing the rate of energy depletion while maintaining higher levels of key metabolites taking part in tricarboxylic acid (TCA) cycle at room temperature storage.

Keywords: Chitosan coating; Citrus grandis L. Osbeck; Energy loss; Granulation stress.