Peroxidase-mimicking evodiamine/indocyanine green nanoliposomes for multimodal imaging-guided theranostics for oral squamous cell carcinoma

Zheng Wei; Huihui Zou; Gongyuan Liu; Chuanhui Song; Chuanchao Tang; Sheng Chen; Guorong Zhang; Jianchuan Ran; Yufeng Wang; Xiteng Yin; Yu Cai; Wei Han

doi:10.1016/j.bioactmat.2020.12.016

Peroxidase-mimicking evodiamine/indocyanine green nanoliposomes for multimodal imaging-guided theranostics for oral squamous cell carcinoma

Bioact Mater. 2021 Jan 13;6(7):2144-2157. doi: 10.1016/j.bioactmat.2020.12.016. eCollection 2021 Jul.

Authors

Zheng Wei^{1

2

3}, Huihui Zou^{1

3}, Gongyuan Liu⁴, Chuanhui Song^{1

3}, Chuanchao Tang^{1

3}, Sheng Chen⁵, Guorong Zhang⁶, Jianchuan Ran^{1

3}, Yufeng Wang^{1

3}, Xiteng Yin^{1

3}, Yu Cai^{7

3}, Wei Han^{1

3}

Affiliations

¹ Department of Oral and Maxillofacial Surgery, Nanjing Stomatological Hospital, Medical School of Nanjing University, 30 Zhongyang Road, 210008, Nanjing, China.
² Pediatric Dentistry, Nanjing Stomatology Hospital, Medical School of Nanjing University, No 30 Zhongyang Road, Nanjing, 210008, China.
³ Central Laboratory of Stomatology, Nanjing Stomatological Hospital, Medical School of Nanjing University, 30 Zhongyang Road, 210008, Nanjing, China.
⁴ Department of Chemistry, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong SAR, China.
⁵ Department of Oral Pathology, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing, China.
⁶ Department of Orthodontics, Nanjing Stomatological Hospital, Medical School of Nanjing University, No 30 Zhongyang Road, Nanjing, 210008, China.
⁷ Clinical Research Institute, Zhejiang Provincial People's Hospital, People's Hospital of Hangzhou Medical College, No. 158 Shangtang Road, Hangzhou, 310014, Zhejiang Province, China.

Abstract

Here, evodiamine (EVO) and the photosensitizer indocyanine green (ICG) were integrated into a liposomal nanoplatform for noninvasive diagnostic imaging and combinatorial therapy against oral squamous cell carcinoma (OSCC). EVO, as an active component extracted from traditional Chinese medicine, not only functioned as an antitumor chemotherapeutic agent but was also capable of ⁶⁸Ga-chelation, thus working as a contrast agent for positron emission tomography/computed tomography (PET/CT) imaging. Moreover, EVO could exhibit peroxidase-like catalytic activity, converting endogenous tumor H₂O₂ into cytotoxic reactive oxygen species (ROS), enabling Chemo catalytic therapy beyond the well-known chemotherapy effect of EVO. As proven by in vitro and in vivo experiments, guided by optical imaging and PET/CT imaging, we show that the theragnostic liposomes have a significant inhibiting effect on in situ tongue tumor through photodynamic therapy combined with chemodynamic chemotherapy.

Keywords: ATCC, American Type Culture Collection; CAT, Catalase Activity; CDT, Chemodynamic therapy; DI water, deionized water; DLS, dynamic light scattering; DMEM, Dulbecco's modified Eagle's medium; EPR, enhanced permeability and retention; EVO, evodiamine; Evodiamine; FBS, fetal bovine serum; FDA, Food and Drug Administration; FI, fluorescence imaging; HRP, horseradish peroxidase; ICG, indocyanine green; NIR, Near-infrared; OSCC, Oral squamous cell carcinoma; Oral squamous cell carcinoma; PBS, polarization beam splitter; PDT, Photodynamic therapy; PET/CT, positron emission tomography/computed tomography; Peroxidase-mimicking; ROS, reactive oxygen species; SD, Sprague-Dawley; SOSG, singlet oxygen sensor green; TEM, transmission electron microscope; THF, tetrahydrofuran; TMB, tetramethylbenzidine; Trimodal antitumor therapy.