Bifidobacterium pseudocatenulatum Ameliorates DSS-Induced Colitis by Maintaining Intestinal Mechanical Barrier, Blocking Proinflammatory Cytokines, Inhibiting TLR4/NF-κB Signaling, and Altering Gut Microbiota

Yang Chen; Bo Yang; Catherine Stanton; R Paul Ross; Jianxin Zhao; Hao Zhang; Wei Chen

doi:10.1021/acs.jafc.0c06329

Bifidobacterium pseudocatenulatum Ameliorates DSS-Induced Colitis by Maintaining Intestinal Mechanical Barrier, Blocking Proinflammatory Cytokines, Inhibiting TLR4/NF-κB Signaling, and Altering Gut Microbiota

J Agric Food Chem. 2021 Feb 10;69(5):1496-1512. doi: 10.1021/acs.jafc.0c06329. Epub 2021 Jan 29.

Authors

Yang Chen^{1

2}, Bo Yang^{1

2

3}, Catherine Stanton^{3

4

5}, R Paul Ross^{3

4

5}, Jianxin Zhao^{1

2}, Hao Zhang^{1

2

6}, Wei Chen^{1

2

6}

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China.
² School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, Jiangsu 214122, China.
³ International Joint Research Laboratory for Pharmabiotics & Antibiotic Resistance, Jiangnan University, Wuxi, Jiangsu 214122, China.
⁴ Teagasc Food Research Centre, Moorepark, Fermoy, Cork P61 C996, Ireland.
⁵ APC Microbiome Ireland, University College Cork, Cork T12 K8AF, Ireland.
⁶ National Engineering Research Center for Functional Food, Jiangnan University, Wuxi, Jiangsu 214122, China.

PMID: 33512996
DOI: 10.1021/acs.jafc.0c06329

Abstract

This study was designed to explore the effects and discrepancy of different CLA-producing Bifidobacterium pseudocatenulatum on relieving colitis and to investigate the potential mechanisms. B. pseudocatenulatum MY40C and CCFM680 were administered to mice with DSS-induced colitis. The content of tight junction proteins and mucin2 was significantly upregulated. TNF-α and IL-6 were downregulated, while IL-10 and PPAR-γ were upregulated. TLR4/NF-κB pathway activation was significantly inhibited. Moreover, each treated strain increased Allobaculum and decreased Sutterella, Bacteroides, and Oscillospira. The colonic conjugated linoleic acid (CLA) concentrations were significantly and positively correlated with the effectiveness of strain in relieving colitis. In conclusion, MY40C and CCFM680 supplementation alleviated DSS-induced colitis by protecting intestinal mechanical barrier, modulating gut microbiota, blocking proinflammatory cytokines, and inhibiting TLR4/NF-κB pathway. These results are conducive to promote clinical trials and product development of probiotics for colitis.

Keywords: Bifidobacterium pseudocatenulatum; colitis; conjugated linoleic acid; gut microbiota; intestinal mechanical barrier.

MeSH terms

Animals
Bifidobacterium pseudocatenulatum / physiology*
Colitis / chemically induced
Colitis / drug therapy*
Colitis / microbiology
Dextran Sulfate / adverse effects
Gastrointestinal Microbiome / drug effects*
Humans
Interleukin-6 / genetics
Interleukin-6 / immunology
Intestinal Mucosa / drug effects*
Intestinal Mucosa / immunology
Male
Mice
Mice, Inbred C57BL
NF-kappa B / genetics
NF-kappa B / immunology*
PPAR gamma / genetics
PPAR gamma / immunology
Probiotics / administration & dosage*
Signal Transduction / drug effects
Toll-Like Receptor 4 / genetics

Substances

Interleukin-6
NF-kappa B
PPAR gamma
Toll-Like Receptor 4
Dextran Sulfate