Potential of Exopolysaccharide from Porphyridium marinum to Contend with Bacterial Proliferation, Biofilm Formation, and Breast Cancer

Mar Drugs. 2021 Jan 27;19(2):66. doi: 10.3390/md19020066.


Exopolysaccharide (EPS) from marine microalgae are promising sources of a new generation of drugs. However, lot of them remain to be discovered and tested. In this study, EPS produced by Porphyridium marinum and its oligomers prepared by High Pressure Homogenizer have been tested for different biological activities, i.e., antibacterial, anti-fungal and antibiofilm activities on Candida albicans, as well as for their effects on the viability of murine breast cancer cells. Results have shown that all EPS samples present some biological activity. For antibacterial and antibiofilm activities, the native EPS exhibited a better efficiency with Minimum Inhibitory Concentration (MIC) from 62.5 µg/mL to 1000 µg/mL depending on the bacterial strain. For Candida albicans, the biofilm formation was reduced by about 90% by using only a 31.3 µg/mL concentration. Concerning breast cancer cells, lower molar masses fractions appeared to be more efficient, with a reduction of viability of up to 55%. Finally, analyses of polymers composition and viscosity measurements were conducted on all samples, in order to propose hypotheses involving the activities caused by the intrinsic properties of polymers.

Keywords: Porphyridium marinum; anti-biofilm activity; anti-cancer activity; antibacterial activity; exopolysaccharide; high pressure homogenizer.

MeSH terms

  • Animals
  • Anti-Bacterial Agents / isolation & purification
  • Anti-Bacterial Agents / pharmacology*
  • Anti-Bacterial Agents / therapeutic use
  • Biofilms / drug effects*
  • Biofilms / growth & development
  • Breast Neoplasms* / drug therapy
  • Cell Line, Tumor
  • Cell Survival / drug effects*
  • Cell Survival / physiology
  • Dose-Response Relationship, Drug
  • Female
  • Mice
  • Microalgae / isolation & purification
  • Microbial Sensitivity Tests / methods
  • Polysaccharides, Bacterial / isolation & purification
  • Polysaccharides, Bacterial / pharmacology*
  • Polysaccharides, Bacterial / therapeutic use
  • Porphyridium* / isolation & purification


  • Anti-Bacterial Agents
  • Polysaccharides, Bacterial