Establishment and characterization of a human urinary bladder carcinoma cell line (TSGH-8301)

J Surg Oncol. 1988 Mar;37(3):177-84. doi: 10.1002/jso.2930370310.

Abstract

A cell line derived from a well-differentiated human transitional cell carcinoma of the urinary bladder, designated TSGH-8301, was established in vitro. The cultured epithelioid cells exhibited monolayer growth and loss of contact inhibition. The tumorigenicity of TSGH-8301 had been shown by growth in soft agar and tumor induction in athymic nude mice. A reverse ratio of lactate dehydrogenase (LHD) isoenzyme in the cell line and nude mouse-grown tumors was seen predominantly with LDH-V. Chromosomal analysis revealed a heterodiploid stem line with a modal number of 50. Sera of urinary bladder cancer patients reacted with membrane antigens of the TSGH-8301 cells, suggesting the existence of tumor-associated antigens in the cells. In vitro chemosensitivity tests of these cells may provide data valuable in the selection of proper anticancer drugs for the TSGH-8301 donor patient.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carcinoma / pathology*
  • Carcinoma / ultrastructure
  • Chromosome Aberrations
  • Humans
  • Isoenzymes
  • L-Lactate Dehydrogenase / analysis
  • Male
  • Mice
  • Microscopy, Electron
  • Middle Aged
  • Neoplasm Transplantation
  • Transplantation, Heterologous
  • Tumor Cells, Cultured / pathology
  • Urinary Bladder Neoplasms / pathology*
  • Urinary Bladder Neoplasms / ultrastructure

Substances

  • Isoenzymes
  • L-Lactate Dehydrogenase