Dammarane-type triterpenoid saponins from Salvia russellii Benth

Phytochemistry. 2021 Apr;184:112653. doi: 10.1016/j.phytochem.2020.112653. Epub 2021 Jan 29.

Abstract

Three undescribed dammarane-type saponins, russelliinosides A-C, together with a common sterol (β-sitosterol), an abietane diterpenoid (18-hydroxyferruginol), two oleane triterpenoids (daturaolone and oleanolic acid), an ursane triterpenoid (ursolic acid) as well as three 5-hydroxyflavones (cirsimaritin, eupatorin, and salvigenin) were isolated from a dichloromethane extract of the aerial parts of Salvia russellii Benth. The chemical structures of the aforementioned compounds were characterized, using detailed spectroscopic analyses, including high-resolution mass spectrometry and 1D and 2D NMR (1H-1H COSY, TOCSY, HSQC, HMBC and NOESY) spectroscopy as well as physicochemical properties. Cytotoxic effects of S. russellii extract and the three isolated russelliinosides were tested against MCF-7 human breast and A549 lung cancer, as well as non-cancer NIH/3T3 cells using MTT reduction assay. Russelliinosides A and B exhibited cytotoxic activities with IC50 values of 7.1 and 30.7 μg/ml against MCF-7 and 33.9 and 69.4 μg/ml against A549 cells, respectively, while russelliinoside C did not show cytotoxicity against cancer cells. On the other hand, russelliinoside A showed an IC50 value of 31.5 μg/ml against NIH/3T3 cells, while russelliinosides B and C had no effect on the viability of these non-cancer cells.

Keywords: Cytotoxic activity; Dammarane triterpenes; Lamiaceae; Russelliinosides; Salvia russellii Benth..

MeSH terms

  • Animals
  • Mice
  • Molecular Structure
  • Oleanolic Acid*
  • Salvia*
  • Saponins*
  • Triterpenes* / pharmacology

Substances

  • Saponins
  • Triterpenes
  • dammarane
  • Oleanolic Acid