Chronic caloric restriction maintains a youthful phosphoproteome in aged skeletal muscle

Mech Ageing Dev. 2021 Apr:195:111443. doi: 10.1016/j.mad.2021.111443. Epub 2021 Jan 30.

Abstract

Caloric restriction (CR) can prolong aged skeletal muscle function, yet the molecular mechanisms are not completely understood. We performed phosphoproteomic analysis on muscle from young and old mice fed an ad libitum diet, and old mice fed a CR diet. CR promoted a youthful phosphoproteomic signature, suppressing several known "pro-aging" pathways including Protein kinase A (PKA). This study validates global signaling changes in skeletal muscle during CR.

Keywords: Aging; Caloric restriction; Mice; PKA; Phosphoproteome.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / physiology*
  • Animals
  • Caloric Restriction / methods*
  • Cyclic AMP-Dependent Protein Kinases / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Muscle, Skeletal* / physiology
  • Muscle, Skeletal* / physiopathology
  • Phosphoproteins / metabolism*
  • Principal Component Analysis / methods
  • Proteomics / methods*
  • Regeneration / physiology
  • Rejuvenation / physiology*
  • Signal Transduction
  • Time

Substances

  • Phosphoproteins
  • Cyclic AMP-Dependent Protein Kinases