Generation and assessment of high-quality mouse oocytes and embryos following nicotinamide mononucleotide administration

STAR Protoc. 2021 Jan 22;2(1):100298. doi: 10.1016/j.xpro.2021.100298. eCollection 2021 Mar 19.


The low quality of oocytes is one of the main causes of the suboptimal reproductive outcome of female mammals with advanced maternal age. Here, we present a detailed protocol to obtain high-quality oocytes and embryos from aged mice by nicotinamide mononucleotide (NMN) administration. We also describe fluorescence staining procedures to assess the organelle dynamics in oocytes, and in vitro fertilization and embryo culture systems to evaluate the influence of NMN on the fertilization ability and embryonic development potential. For complete information on the use and execution of this protocol, please refer to Miao et al. (2020).

Keywords: Cell culture; Cell isolation; Microscopy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / metabolism*
  • Embryo Culture Techniques
  • Embryonic Development / drug effects*
  • Female
  • Fertilization in Vitro
  • Mice
  • Nicotinamide Mononucleotide / pharmacology*
  • Oocytes / metabolism*


  • Nicotinamide Mononucleotide