Neutralization of SARS-CoV-2 spike 69/70 deletion, E484K, and N501Y variants by BNT162b2 vaccine-elicited sera

Xuping Xie; Yang Liu; Jianying Liu; Xianwen Zhang; Jing Zou; Camila R Fontes-Garfias; Hongjie Xia; Kena A Swanson; Mark Cutler; David Cooper; Vineet D Menachery; Scott Weaver; Philip R Dormitzer; Pei-Yong Shi

doi:10.1101/2021.01.27.427998

Neutralization of SARS-CoV-2 spike 69/70 deletion, E484K, and N501Y variants by BNT162b2 vaccine-elicited sera

bioRxiv [Preprint]. 2021 Jan 27:2021.01.27.427998. doi: 10.1101/2021.01.27.427998.

Authors

Xuping Xie¹, Yang Liu¹, Jianying Liu^{2

3}, Xianwen Zhang¹, Jing Zou¹, Camila R Fontes-Garfias¹, Hongjie Xia¹, Kena A Swanson⁴, Mark Cutler⁴, David Cooper⁴, Vineet D Menachery^{2

3}, Scott Weaver^{2

3}, Philip R Dormitzer⁴, Pei-Yong Shi^{1

3

5

6

7}

Affiliations

¹ Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston TX, U.S.A.
² Departments of Microbiology and Immunology, University of Texas Medical Branch, Galveston TX, U.S.A.
³ Institute for Human Infection and Immunity, University of Texas Medical Branch, Galveston, TX, U.S.A.
⁴ Pfizer, 401 N Middletown Rd., Pearl River, NY 10960, U.S.A.
⁵ Institute for Translational Sciences, University of Texas Medical Branch, Galveston, TX, U.S.A.
⁶ Sealy Institute for Vaccine Sciences, University of Texas Medical Branch, Galveston, TX, U.S.A.
⁷ Sealy Center for Structural Biology & Molecular Biophysics, University of Texas Medical Branch, Galveston, TX, U.S.A.

Abstract

We engineered three SARS-CoV-2 viruses containing key spike mutations from the newly emerged United Kingdom (UK) and South African (SA) variants: N501Y from UK and SA; 69/70-deletion+N501Y+D614G from UK; and E484K+N501Y+D614G from SA. Neutralization geometric mean titers (GMTs) of twenty BTN162b2 vaccine-elicited human sera against the three mutant viruses were 0.81- to 1.46-fold of the GMTs against parental virus, indicating small effects of these mutations on neutralization by sera elicited by two BNT162b2 doses.

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Preprint

Abstract

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