Effects of Sodium Butyrate and 3-aminobenzamide on Survival of Chinese Hamster HA-1 Cells After X Irradiation

Radiat Res. 1988 Apr;114(1):186-91.

Abstract

HA-1 cells were grown in medium containing 2 mM sodium butyrate and then exposed to graded doses of 250 kVp X rays. After irradiation, some of the butyrate-treated cultures were treated with either 10 or 20 mM 3-aminobenzamide for 2 h at 37 degrees C. The butyrate treatment produced a small degree of radiation sensitization as indicated by an increase in the alpha parameter using a linear-quadratic description of survival responses. The dose-modifying factor at the 10% survival level (DMF10) was 1.15. Similarly, both 10 and 20 mM 3-aminobenzamide treatments produced concentration-dependent increases in radiosensitization, again as indicated by an increase in the value of the alpha constant, with DMF10 values of 1.22 and 1.40, respectively. However, the combination of the 2 mM sodium butyrate + 10 mM 3-aminobenzamide treatments produced a supraadditive response in terms of increased cell killing (DMF10 = 1.76). We interpret this to mean that 3-aminobenzamide inhibits a sodium butyrate associated increase in poly(ADP-ribose) which then predisposes hyperacetylated chromatin to attack by endogenous nucleases leading to increased cytotoxicity.

MeSH terms

  • Animals
  • Benzamides / pharmacology*
  • Butyrates / pharmacology*
  • Butyric Acid
  • Cell Line
  • Cell Survival / drug effects
  • Cell Survival / radiation effects*
  • Cricetinae
  • Cricetulus
  • In Vitro Techniques
  • Radiation-Sensitizing Agents / pharmacology

Substances

  • Benzamides
  • Butyrates
  • Radiation-Sensitizing Agents
  • Butyric Acid
  • 3-aminobenzamide