High carbohydrate diet ingestion increases post-meal lipid synthesis and drives respiratory exchange ratios above 1

Abstract

Locusts have been reported to elevate metabolic rate in response to high carbohydrate diets; this conclusion was based on metabolic rates calculated from CO₂ production, a common practice for insects. However, respiratory exchange ratio (RER, CO₂ production divided by O₂ consumption) can rise above 1 as a result of de novo lipid synthesis, providing an alternative possible explanation of the prior findings. We studied the relationship between macronutrient ingestion, RER and lipid synthesis using South American locusts (Schistocerca cancellata) reared on artificial diets varying in protein:carbohydrate (p:c) ratio. RER increased and rose above 1 as dietary p:c ratio decreased. Lipid accumulation rates were strongly positively correlated with dietary carbohydrate content and ingestion. RERs above 1 were only observed for animals without food in the respirometry chamber, suggesting that hormonal changes after a meal may drive lipid synthesis. Schistocerca cancellata does not elevate metabolic rate on low p:c diets; in fact, the opposite trend was observed.

Keywords: Carbohydrates; De novo lipogenesis; Locusts; Macronutrients; Respiratory exchange ratio; Respiratory quotient.