A wheat bran inducible expression system for the efficient production of α-L-arabinofuranosidase in Bacillus subtilis

Enzyme Microb Technol. 2021 Mar:144:109726. doi: 10.1016/j.enzmictec.2020.109726. Epub 2020 Dec 29.

Abstract

α-l-arabinofuranosidases (EC 3.2.1.55; AFs) cause the release of arabinosyl residues from hemicellulose polymers such as xylans, and are receiving increased levels of research attention as they could be applied in a range of processes that involve the enzymatic degradation of xylans. The secretory production of bacterial AFs has not been attempted previously. In this study, we designed a unique induction system for the production of a recombinant AF in Bacillus subtilis in order to exploit its enzymic degradation of wheat bran. We found that non-starch phytochemicals were more efficient than d-xylose when inducing the expression of T7 RNA polymerase and driving the transcription of AF by the T7 promoter. The host cell, B. subtilis (ATCC 6051a-derived strain 164T7P) was engineered to incorporate a DNA cassette that expressed T7 RNA polymerase under the control of a d-xylose inducible promoter (PxylA). The T7 promoter engineered into 164T7P was initially tested and compared with P43 in terms of GFP expression; we found that the expression level of GFP by the T7 promoter was ten-fold higher than that achieved by P43. When cultured in a flask with gentle shaking, and with d-xylose as an inducer, the recombinant strain successfully expressed arbf, a family 51 (GH 51) glycoside hydrolase from Bacillus licheniformis, and secreted 141.4 ± 4.8 U/mL of enzyme, with a Km of 1.4 ± 0.1 mM and a kcat of 139.4 s-1. However, the protein was devoid of a secretary signal peptide. When cultures were supplemented with wheat bran, the maximal yield of the secreted AF reached 194.8 ± 4.1 U/mL. The results provide a foundation for the high level production of heterologous proteins using wheat bran as the inducer in B. subtilis.

Keywords: Bacillus subtilis; Hemicellulose; T7 expression system; Xylan; α-l-arabinofuranosidase.

MeSH terms

  • Bacillus subtilis* / genetics
  • Bacillus subtilis* / metabolism
  • Dietary Fiber*
  • Glycoside Hydrolases / genetics
  • Glycoside Hydrolases / metabolism
  • Promoter Regions, Genetic
  • Recombinant Proteins / genetics
  • Xylose

Substances

  • Dietary Fiber
  • Recombinant Proteins
  • Xylose
  • Glycoside Hydrolases