Quantitative analysis of an impact of P-glycoprotein on edoxaban's disposition using a human physiologically based pharmacokinetic (PBPK) model

Int J Pharm. 2021 Mar 15:597:120349. doi: 10.1016/j.ijpharm.2021.120349. Epub 2021 Feb 2.

Abstract

The purpose of this study was to evaluate the impact of P-glycoprotein (P-gp) efflux on edoxaban absorption in gastrointestinal tracts quantitatively by a physiologically based pharmacokinetic (PBPK) model constructed with clinical and non-clinical observations (using GastroPlus™ software). An absorption process was described by the advanced compartmental absorption and transit model with the P-gp function. A human PBPK model was constructed by integrating the clinical and non-clinical observations. The constructed model was demonstrated to reproduce the data observed in the mass-balance study. Thus, elimination pathways can be quantitatively incorporated into the model. A constructed model successfully described the difference in slopes of plasma concentration (Cp)-time curve at around 8 - 24 hr post-dose between intravenous infusion and oral administration. Furthermore, the model without P-gp efflux activity can reproduce the Cp-time profile in the absence of P-gp activity observed from the clinical DDI study results. Since the difference of slopes between intravenous infusion and oral administration also disappeared by the absence of P-gp efflux activity, P-gp must be a key molecule to govern edoxaban's PK behavior. The constructed PBPK model will help us to understand the significant contribution of P-gp in edoxaban's disposition in gastrointestinal tracts quantitatively.

Keywords: Advanced compartmental absorption and transit (ACAT) model; Drug-drug interactions (DDI); GastroPlus™; P-glycoprotein (P-gp); Physiologically based pharmacokinetic (PBPK) model.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B, Member 1* / metabolism
  • Biological Transport
  • Humans
  • Models, Biological
  • Pyridines*
  • Thiazoles

Substances

  • ATP Binding Cassette Transporter, Subfamily B, Member 1
  • Pyridines
  • Thiazoles
  • edoxaban