Dopamine controls neuronal spontaneous calcium oscillations via astrocytic signal

Cell Calcium. 2021 Mar:94:102359. doi: 10.1016/j.ceca.2021.102359. Epub 2021 Jan 29.

Abstract

Dopamine is a neuromodulator and neurotransmitter responsible for a number of physiological processes. Dysfunctions of the dopamine metabolism and signalling are associated with neurological and psychiatric diseases. Here we report that in primary co-culture of neurons and astrocytes dopamine-induces calcium signal in astrocytes and suppress spontaneous synchronous calcium oscillations (SSCO) in neurons. Effect of dopamine on SSCO in neurons was dependent on calcium signal in astrocytes and could be modified by inhibition of dopamine-induced calcium signal or by stimulation of astrocytic calcium rise with ATP. Ability of dopamine to suppress SSCO in neurons was independent on D1- or D2- like receptors but dependent on GABA and alpha-adrenoreceptors. Inhibitor of monoaminoxidase bifemelane blocked effect of dopamine on astrocytes but also inhibited the effect dopamine on SSCO in neurons. These findings suggest that dopamine-induced calcium signal may stimulate release of neuromodulators such as GABA and adrenaline and thus suppress spontaneous calcium oscillations in neurons.

Keywords: Astrocyte; Calcium; Dopamine; Neuron.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Astrocytes / drug effects
  • Astrocytes / metabolism*
  • Calcium Signaling* / drug effects
  • Cells, Cultured
  • Chloride Channels / metabolism
  • Dopamine / metabolism*
  • Dopamine Agonists / pharmacology
  • GABA Antagonists / pharmacology
  • Ion Channel Gating / drug effects
  • Male
  • Neurons / drug effects
  • Neurons / metabolism*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Adrenergic / metabolism
  • Receptors, Dopamine D1 / metabolism
  • Receptors, Dopamine D2 / metabolism
  • Receptors, GABA / metabolism

Substances

  • Chloride Channels
  • Dopamine Agonists
  • GABA Antagonists
  • Receptors, Adrenergic
  • Receptors, Dopamine D1
  • Receptors, Dopamine D2
  • Receptors, GABA
  • Adenosine Triphosphate
  • Dopamine