Protective effect of liraglutide on experimental testicular ischaemia reperfusion in rats

Andrologia. 2021 May;53(4):e14000. doi: 10.1111/and.14000. Epub 2021 Feb 6.


This study was performed to evaluate the effect of liraglutide on experimental testicular ischaemia reperfusion in rats in terms of biochemistry, histopathology and immunohistochemistry. A total of 28 male Wistar-Albino rats were divided randomly into 4 groups: control (7), sham (7), ischaemia-reperfusion (7) and ischaemia-reperfusion + liraglutide (7). Biochemically, Nitric Oxide, Malondialdehyde, Superoxide dismutase, Glutathione peroxidase and Catalase levels were measured in the testis. Apoptosis protease activating factor-1 and inducible nitric oxide synthase activity were evaluated immunohistochemically as well. Statistical analyses were made via the Kruskal-Wallis and Mann-Whitney U tests. In the reperfusion group, CAT and SOD values were increased (p > .05), NO and MDA values were decreased (p < .05) after administration of liraglutide. In addition, GPx values were significantly increased in ischaemia reperfusion + liraglutide administered group compared to reperfusion group (p < .05). Apaf-1 and iNOS activity were significantly decreased with the addition of liraglutide treatment to the ischaemia-reperfusion group (p < .05). First of all, we would like to say that liraglutide treatment is moderately preventive against I/R injury in testicular torsion. The anti-inflammatory, antioxidant and antiapoptotic properties of liraglutide are create a moderately protective effect as we show in this study.

Keywords: ischaemia-reperfusion; liraglutide; oxidative stress; testicular torsion.

MeSH terms

  • Animals
  • Humans
  • Ischemia
  • Liraglutide / metabolism
  • Liraglutide / pharmacology
  • Liraglutide / therapeutic use
  • Male
  • Malondialdehyde / metabolism
  • Oxidative Stress
  • Rats
  • Rats, Wistar
  • Reperfusion
  • Reperfusion Injury* / metabolism
  • Reperfusion Injury* / prevention & control
  • Spermatic Cord Torsion* / metabolism
  • Superoxide Dismutase / metabolism
  • Testis / metabolism


  • Malondialdehyde
  • Liraglutide
  • Superoxide Dismutase