Effect of a genetic polymorphism in SREBP1 on fatty acid composition and related gene expression in subcutaneous fat tissue of beef cattle breeds

Anim Sci J. 2021 Jan-Dec;92(1):e13521. doi: 10.1111/asj.13521.

Abstract

Sterol regulatory element-binding factor 1 (SREBP1) plays an important role in the lipogenesis which affects fatty acid (FA) composition in backfat and consequently influences beef nutritional quality. This study analyzed the association of 84 bp-indel, both short (S) and long (L) alleles in intron 5 of SREBP1, with FA composition and gene expression of SREBP1 in backfat of northern Spanish beef breeds (Pirenaica, Salers and Holstein-Friesian). Phylogenetic analysis suggests that 84 bp-indel of ruminants is a highly conserved region compared with those in the full-length sequence of intron 5 or mRNA of SREBP1 among species. Overall, higher content of polyunsaturated FAs was observed in SL genotype compared to LL genotype of 84 bp-Indel (p < .05). In particular, in Pirenaica, SL genotype was associated with a higher content of stearic (18:0), α-linolenic (18:3n-3) acid, and total n-3 content (p < .05). However, the gene expression of SREBP1 did not differ among genotypes of 84 bp-Indel (p > .05).

Keywords: beef; fatty acid composition; gene expression; genetic polymorphism; meat quality.

MeSH terms

  • Alleles
  • Animals
  • Back
  • Cattle / genetics*
  • Cattle / metabolism*
  • Fatty Acids / metabolism*
  • Female
  • Gene Expression*
  • Genotype
  • Introns / genetics
  • Nutritive Value
  • Phylogeny
  • Polymorphism, Genetic / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Red Meat
  • Sterol Regulatory Element Binding Protein 1 / genetics*
  • Sterol Regulatory Element Binding Protein 1 / metabolism*
  • Subcutaneous Fat / metabolism*

Substances

  • Fatty Acids
  • RNA, Messenger
  • Sterol Regulatory Element Binding Protein 1