Objective: To study the impact of anticoagulant to the quality of umbilical cord blood (UCB).
Methods: 6060 cord blood units (CBUs) were classified into five groups, such as 28 ml: (10-29) ml, 28 ml: (30-69) ml, 28 ml: (70-109) ml, 28 ml: (110-150) ml and 28 ml: (>150) ml according to volume ratio of anticoagulant and CBVs. The count of pre-cryopreservation total nucleated cell (pre-TNC), the viability of nucleated cell (VNC), the amount of CFU-GM and the ratio changes of CD34+ were evaluated and analyzed statistically.
Results: It was found that pre-TNC increased with the growth of volume of CBUs (r=0.9937) under the certain volume of antico-agulant, and the TNC in the minimum UCB volume group was (2.57±0.89)×108; the VNC grew up with the increasing count viability of volume (r=0.9897), and the average viability of the minimum volume group remained over 95%; the CFU-GM climbed up with the increasing of volume (r=0.9024), and the number of CFV-GM in minimum volume group reached to of 89/×105; CD34+% grew up with the increase of volume of CBUs (r=0.9641), and the ratio was (0.30±0.19)% for the minimum volume group.
Conclusion: In certain volume of anticoagulant in collection-bag, pre-TNC, VNC, CFU-GM and CD34+% are all dropped with the decrease of CBUs volume , however, all above-mentioned indexes in the minimun random group still meet the requirement for clinical administration.
题目: 固定抗凝剂量加入不同量脐血后脐血质量分析评估的研究.
目的: 研究固定抗凝剂量加入不同量脐血后对脐血质量的影响.
方法: 对6060份脐血样本，按照抗凝剂/脐血体积比分为28：（10-29）ml组、28：（30-69）ml组、28：（70-109）ml组、28:（110-150）ml组和28：（>150）ml 组。分析各组脐血的冻前有核细胞数、有核细胞活性、CFU-GM数量、CD34+细胞比例的变化情况，对各项指标进行统计学分析.
结果: 在抗凝剂量不变的情况下，冻前有核细胞数量随着脐血体积增加而上升（r=0.9937），最小脐血量组有核细胞数量达到（2.57±0.89）×108。有核细胞活性随着脐血体积增加而上升（r=0.9897），最小脐血量组冻前有核细胞活性的均值仍保持在95%以上。CFU-GM/×105集落数量随着脐血体积增加而上升（r=0.9024），最小脐血量组的CFU-GM/×105集落数量达到89个。CD34+细胞比例随着脐血体积增加而上升（r=0.9641），最小脐血量组的CD34+细胞比例为（0.30±0.19）.
结论: 在抗凝剂体积不变的情况下，随着采集血量的降低，脐血冻前有核细胞数、有核细胞活性、CFU-GM集落数和CD34+细胞比例也相应降低，但最小血量组的上述指标均可满足临床需求.