cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins

Chem Commun (Camb). 2021 Mar 7;57(19):2416-2419. doi: 10.1039/d0cc07541h. Epub 2021 Feb 8.


We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.

MeSH terms

  • Antibodies, Monoclonal / chemistry*
  • Biosensing Techniques
  • DNA, Complementary / chemistry*
  • Humans
  • Peptide Library
  • Protein Binding
  • Puromycin / chemistry
  • RNA, Messenger / chemistry
  • Ribonucleases / chemistry
  • Sensitivity and Specificity


  • Antibodies, Monoclonal
  • DNA, Complementary
  • Peptide Library
  • RNA, Messenger
  • Puromycin
  • Ribonucleases