The effect of inosine on the post ischemic heart as bio-energy recovering factor in 31P-MRS

Biochem Biophys Res Commun. 1988 Mar 30;151(3):1408-15. doi: 10.1016/s0006-291x(88)80519-9.


Perfused guinea-pig hearts, which were analyzed by 31P-MRS, were subjected to 30 and 60 minute ischemia and reperfused using two perfusates, one containing 200 microM inosine, and the other without inosine. After 4 hour reperfusion with inosine, ATP levels increased to 95.5% of preischemic value (30 minute ischemia) and 76.2% (60 minute ischemia). However, after 4 hour reperfusion without inosine, ATP levels increased only to 72.2% (30 minute ischemia) and to 48.2% (60 minute ischemia). In 60 minute ischemic hearts reperfused with inosine, left ventricular maximal positive dp/dt (LV dp/dt) was improved significantly to 82.4% after 6 hour reperfusion in contrast to hearts reperfused without inosine (43.1%). Administration of inosine was very useful for increasing myocardial gross energy product and improving cardiac performance.

MeSH terms

  • Adenosine Triphosphate / metabolism
  • Animals
  • Coronary Disease / physiopathology*
  • Energy Metabolism / drug effects*
  • Guinea Pigs
  • Heart / drug effects*
  • Hydrogen-Ion Concentration
  • Inosine / pharmacology*
  • Magnetic Resonance Spectroscopy
  • Male
  • Perfusion
  • Phosphocreatine / metabolism
  • Time Factors


  • Phosphocreatine
  • Inosine
  • Adenosine Triphosphate