Evaluation of active matrix metalloproteinase-8 (aMMP-8) chair-side test as a diagnostic biomarker in the staging of periodontal diseases

Arch Oral Biol. 2021 Apr;124:104955. doi: 10.1016/j.archoralbio.2020.104955. Epub 2021 Jan 20.


Objective: There is a need for a reliable complementary diagnostic tool that ideally helps to screen, differentiate sites, activities of and predict future periodontal tissue destruction. The purpose of this cross-sectional study was to investigate the screening and prevention potential of the chair-side/point-of-care (PoC) diagnostic test of salivary active matrix metalloproteinase-8 (aMMP-8) levels at different stages of periodontal disease and periodontal health.

Material & methods: 80 individuals were included in this study; 18 with periodontitis stage 3 (P-Stage III), 19 with periodontitis stage-4 (P-Stage IV), 21 with gingivitis, and 22 with clinically healthy periodontium (H). The aMMP-8 levels in GCF and saliva were analyzed by chairside point-of-care aMMP-8 lateral flow immunotest and also by a time-resolved immunofluorescence assay (IFMA).

Results: The sensitivity of the chair-side/PoC test was 83.9 % while specificity was 79.2 %. The aMMP-8 IFMA levels in GCF were significantly higher in P-Stage IV group than P-Stage III, gingivitis and healthy groups (p = 0.01, p = 0.001, p = 0.00, respectively). Moreover, P-Stage III and gingivitis groups had significantly higher aMMP-8 IFMA levels than the healthy group (p < 0.05).

Conclusion: The aMMP-8 chair-side test showed promising results in its ability to recognize and predict the inflammatory status even at the very initial/early stages. aMMP-8 chair-side test could be a valuable adjunctive diagnostic and preventive tool to conventional clinical methods in detecting periodontal disease.

Keywords: Chair-side test; Gingival crevicular fluid; Matrix metalloproteinase 8; Periodontal diseases.

MeSH terms

  • Biomarkers / analysis
  • Cross-Sectional Studies
  • Gingival Crevicular Fluid / chemistry
  • Gingivitis* / diagnosis
  • Humans
  • Matrix Metalloproteinase 8
  • Periodontal Diseases* / diagnosis


  • Biomarkers
  • MMP8 protein, human
  • Matrix Metalloproteinase 8