The N- and O-linked carbohydrate chains of human, bovine and porcine plasminogen. Species specificity in relation to sialylation and fucosylation patterns

Eur J Biochem. 1988 Apr 5;173(1):57-63. doi: 10.1111/j.1432-1033.1988.tb13966.x.


The structures of the N- and O-glycans of human, bovine and porcine plasminogen were determined by 500-MHz 1H-NMR spectroscopy. The N-glycans of all three species proved to be of the N-acetyllactosamine type differing from one another with respect to the sialylation and fucosylation patterns. In the N-glycan of human plasminogen the two antennae are sialylated with N-acetylneuraminic acid (NeuAc), whereas in the bovine counterpart both branches carry significant amounts of N-glycolylneuraminic acid (NeuGc). In porcine plasminogen the sialic acid is mainly NeuAc; the Man alpha 1----6 branch, however, is only partially sialylated. In addition, the porcine N-glycan is fucosylated to about 80% in alpha 1----6 linkage to the GlcNAc-1 residue. The O-glycans of the three species possess an identical Gal beta 1----3GalNAc core which is alpha 2----3 sialylated with NeuAc at Gal. The disialylated form, which is also present in all three species, has an additional NeuAc residue in alpha 2----6 linkage to GalNAc. Mono- and disialylated forms occur in different molar ratios in the different plasminogens: 80:20 in human, 70:30 in bovine and 50:50 in porcine. This study on the carbohydrate moiety of these three plasminogens reveals species specificity in terms of various types of microheterogeneities.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carbohydrate Conformation
  • Cattle
  • Fucose / metabolism*
  • Humans
  • N-Acetylneuraminic Acid
  • Neuraminic Acids
  • Plasminogen* / metabolism
  • Polysaccharides* / isolation & purification
  • Sialic Acids / metabolism*
  • Species Specificity*
  • Structure-Activity Relationship
  • Swine


  • Neuraminic Acids
  • Polysaccharides
  • Sialic Acids
  • N-glycolylneuraminic acid
  • Fucose
  • Plasminogen
  • N-Acetylneuraminic Acid