Rab14/MACF2 complex regulates endosomal targeting during cytokinesis

Mol Biol Cell. 2021 Apr 1;32(7):554-566. doi: 10.1091/mbc.E20-09-0607. Epub 2021 Feb 10.


Abscission is a complex cellular process that is required for mitotic division. It is well established that coordinated and localized changes in actin and microtubule dynamics are vital for cytokinetic ring formation, as well as establishment of the abscission site. Actin cytoskeleton reorganization during abscission would not be possible without the interplay between Rab11- and Rab35-containing endosomes and their effector proteins, whose roles in regulating endocytic pathways at the cleavage furrow have now been studied extensively. Here, we identified Rab14 as a novel regulator of cytokinesis. We demonstrate that depletion of Rab14 causes either cytokinesis failure or significantly prolongs division time. We show that Rab14 contributes to the efficiency of recruiting Rab11-endosomes to the thin intracellular bridge (ICB) microtubules and that Rab14 knockout leads to inhibition of actin clearance at the abscission site. Finally, we demonstrate that Rab14 binds to microtubule minus-end interacting MACF2/CAMSAP3 complex and that this binding affects targeting of endosomes to the ICB microtubules. Collectively, our data identified Rab14 and MACF2/CAMSAP3 as proteins that regulate actin depolymerization and endosome targeting during cytokinesis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Actins / metabolism
  • Cytokinesis / physiology*
  • Endosomal Sorting Complexes Required for Transport / metabolism
  • Endosomes / metabolism
  • Endosomes / physiology
  • HeLa Cells
  • Humans
  • Microtubule-Associated Proteins / metabolism*
  • Microtubule-Associated Proteins / physiology
  • Microtubules / physiology
  • rab GTP-Binding Proteins / metabolism*
  • rab GTP-Binding Proteins / physiology


  • Actins
  • Camsap3 protein, human
  • Endosomal Sorting Complexes Required for Transport
  • Microtubule-Associated Proteins
  • Rab14 protein, human
  • rab11 protein
  • rab GTP-Binding Proteins