Vancomycin-resistant Enterococcus faecium in Algeria: phenotypic and genotypic characterization of clinical isolates

J Infect Dev Ctries. 2021 Jan 31;15(1):95-101. doi: 10.3855/jidc.12482.

Abstract

Introduction: vancomycin-resistant Enterococcus faecium (VREfm) is a major public health problem worldwide. The aim of our study was to determine the microbiological, epidemiological and molecular characteristics of VREfm isolated in north-central, eastern and western Algeria.

Methodology: a collection of 48 VREfm isolated from September 2010 to April 2017 in several Algerian hospitals were studied. Minimum inhibitory concentrations (MICs) were determined by E-test method according to CLSI guidelines. the detection of van genotype of all strains was performed by PCR. Clonal relationship of five VREfm targeted by region were characterized using multilocus sequence typing (MLST).

Results: All isolates have multidrug-resistance (MDR) and were resistant to at least five classes of antibiotics; however, all were susceptible to tigecycline and daptomycin with MIC50 at 0.094 µg/mL and 2 µg/mL respectively. All strains belonged to vanA genotype and have high level of resistance to vancomycin and teicoplanin. MLST revealed two sequence types (STs): ST80 (from the four regions of Algeria) and ST789, both belonging to the former hospital-adapted clonal complex CC17.

Conclusions: the alarming dissemination of MDR E. faecium vanA and the ST80 in several regions of Algeria suggest a clonal spread of VREfm strains, which urgently require implementation of adequate infection control measures.

Keywords: Algeria; E. faecium; Enterococcus faecium; MLST; antimicrobial susceptibility; vanA; vancomycin resistant.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Algeria / epidemiology
  • Anti-Bacterial Agents / pharmacology*
  • Child
  • Child, Preschool
  • Cross Infection / microbiology
  • Daptomycin / pharmacology
  • Drug Resistance, Multiple, Bacterial
  • Enterococcus faecium / drug effects*
  • Enterococcus faecium / genetics*
  • Enterococcus faecium / isolation & purification
  • Female
  • Genotype
  • Gram-Positive Bacterial Infections / microbiology*
  • Hospitals
  • Humans
  • Infant
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Molecular Epidemiology
  • Multilocus Sequence Typing
  • Teicoplanin / pharmacology
  • Tigecycline / pharmacology
  • Vancomycin / pharmacology*
  • Vancomycin Resistance
  • Vancomycin-Resistant Enterococci / drug effects*
  • Vancomycin-Resistant Enterococci / genetics*
  • Vancomycin-Resistant Enterococci / isolation & purification
  • Virulence Factors
  • Young Adult

Substances

  • Anti-Bacterial Agents
  • Virulence Factors
  • Teicoplanin
  • Vancomycin
  • Tigecycline
  • Daptomycin