Exploitation of Vitis vinifera, Foeniculum vulgare, Cannabis sativa and Punica granatum By-Product Seeds as Dermo-Cosmetic Agents

Molecules. 2021 Jan 31;26(3):731. doi: 10.3390/molecules26030731.


In the current study, by-product seed pastes (VSPs) from Vitis vinifera, Foeniculum vulgare, Cannabis sativa and Punica granatum, generated during the oil production process, were investigated for their potential exploitation as dermo-cosmetic agent. The extraction pipeline of all the raw materials was developed with emphasis on green methodologies and employed on laboratory scale based on industry-adopted techniques. Two different protocols were applied, Supercritical Fluid Extraction (SFE) and Ultrasound Assisted Extraction (UAE); the by-product pastes were defatted with supercritical CO2 and n-Hexane, respectively. Then, two SFE extracts (CO2 with 10% and 20% of ethanol as co-solvent) and two UAE extracts (with ethanol and ethanol/water 1:1 v/v) were obtained from each raw material. The providing yield range was between 2.6 to 76.3 mg/g raw material. The extracts were analyzed with High-Performance Liquid Chromatography coupled with Diode Array Detector (HPLC-DAD) and Liquid Chromatography coupled with High-Resolution Mass Spectrometer (LC-HRMS), and the major compounds, were identified. All the extracts were evaluated for their antioxidant and inhibition activity against collagenase, elastase and tyrosinase enzymes. Grapevine by-product extracts found rich in proanthocyanidins and presented the higher inhibition activity. A holistic green experimental methodology is proposed for the obtainment of extracts from significant medicinal plants by-products that provides us with promising results concerning dermo-cosmetic properties, especially for grape seeds extracts.

Keywords: anti-collagenase; anti-elastase; anti-tyrosinase; fennel; grapevine; hemp; pomegranate; seed by-products; supercritical fluid extraction; ultrasound extraction.

MeSH terms

  • Aging / drug effects
  • Cannabis / chemistry*
  • Cosmetics / pharmacology*
  • Enzyme Inhibitors / pharmacology
  • Foeniculum / chemistry*
  • Plant Extracts / pharmacology*
  • Pomegranate / chemistry*
  • Seeds / chemistry
  • Skin / drug effects*
  • Vitis / chemistry*


  • Cosmetics
  • Enzyme Inhibitors
  • Plant Extracts