Diagnosis of respiratory syncytial virus and influenza A and B with cobas® Liat® from nasopharyngeal aspirations in pediatrics

Diagn Microbiol Infect Dis. 2021 Jun;100(2):115326. doi: 10.1016/j.diagmicrobio.2021.115326. Epub 2021 Feb 1.

Abstract

The cobas® Liat® Influenza A/B and respiratory syncytial virus assay was tested on nasopharyngeal aspirates. The resolution of invalid samples was performed using a preanalytical step. cobas® Liat® can be used on nasopharyngeal aspirates with a preanalytical processing step, with a slightly diminished performances in detecting respiratory syncytial virus but not for influenza.

Keywords: Influenza; Molecular diagnosis; Point-of-care testing; Polymerase chain reaction; Respiratory syncytial virus; Respiratory viruses.

MeSH terms

  • Child, Preschool
  • Female
  • Humans
  • Infant
  • Infant, Newborn
  • Influenza A virus / isolation & purification*
  • Influenza B virus / isolation & purification*
  • Influenza, Human / diagnosis*
  • Male
  • Nasopharynx / virology*
  • Point-of-Care Testing
  • Respiratory Syncytial Virus Infections / diagnosis*
  • Respiratory Syncytial Virus Infections / virology
  • Respiratory Syncytial Viruses / isolation & purification*
  • Reverse Transcriptase Polymerase Chain Reaction / methods