LncRNA-WAS and lncRNA-C8807 interact with miR-142a-3p to regulate the inflammatory response in grass carp

Kun Fan; Yubang Shen; Xiaoyan Xu; Lizhu Tao; Tianjie Bao; Jiale Li

doi:10.1016/j.fsi.2021.02.003

LncRNA-WAS and lncRNA-C8807 interact with miR-142a-3p to regulate the inflammatory response in grass carp

Fish Shellfish Immunol. 2021 Apr:111:201-207. doi: 10.1016/j.fsi.2021.02.003. Epub 2021 Feb 11.

Authors

Kun Fan¹, Yubang Shen², Xiaoyan Xu³, Lizhu Tao¹, Tianjie Bao¹, Jiale Li⁴

Affiliations

¹ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China.
² National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China.
³ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China. Electronic address: xyxu@shou.edu.cn.
⁴ Key Laboratory of Freshwater Aquatic Genetic Resources Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai, China; Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, China. Electronic address: jlli2009@126.com.

PMID: 33582280
DOI: 10.1016/j.fsi.2021.02.003

Abstract

Septicemia of grass carp is a systemic inflammatory reaction caused by bacterial infection. More and more evidences show that long non-coding RNAs (lncRNAs) can participate in the regulation of inflammatory response. In the present study, lncRNA-WAS and lncRNA-C8807 were confirmed to be involved in the inflammatory response following infection with Aeromonas hydrophila. LncRNA-WAS and lncRNA-C8807 could interact with miR-142a-3p. LncRNA-WAS and lncRNA-C8807 interact with miR-142a-3p to effect pro-inflammatory genes and NF-κB pathway. Our results provide a theoretical basis for studying the molecular mechanism underlying the regulation of inflammation by lncRNA in grass carp.

Keywords: Aeromonas hydrophila; Ctenopharyngodon idella; Innate immunity; lncRNA-C8807; lncRNA-WAS; miR-142a-3p.

MeSH terms

Aeromonas hydrophila / physiology
Animals
Carps / immunology*
Fish Diseases / genetics
Fish Diseases / immunology*
Fish Diseases / microbiology
Gene Expression Regulation / immunology*
Gram-Negative Bacterial Infections / genetics
Gram-Negative Bacterial Infections / immunology
Gram-Negative Bacterial Infections / microbiology
Gram-Negative Bacterial Infections / veterinary
Immunity, Innate*
Inflammation / genetics
Inflammation / immunology
Inflammation / microbiology
Inflammation / veterinary
MicroRNAs / immunology*
RNA, Long Noncoding / immunology*

Substances

MicroRNAs
RNA, Long Noncoding