Plant Factories Are Heating Up: Hunting for the Best Combination of Light Intensity, Air Temperature and Root-Zone Temperature in Lettuce Production

Laura Carotti; Luuk Graamans; Federico Puksic; Michele Butturini; Esther Meinen; Ep Heuvelink; Cecilia Stanghellini

doi:10.3389/fpls.2020.592171

Plant Factories Are Heating Up: Hunting for the Best Combination of Light Intensity, Air Temperature and Root-Zone Temperature in Lettuce Production

Front Plant Sci. 2021 Jan 28:11:592171. doi: 10.3389/fpls.2020.592171. eCollection 2020.

Authors

Laura Carotti¹, Luuk Graamans², Federico Puksic³, Michele Butturini³, Esther Meinen², Ep Heuvelink³, Cecilia Stanghellini²

Affiliations

¹ Department of Biological, Geological and Environmental Sciences, Alma Mater Studiorum, University of Bologna, Bologna, Italy.
² Greenhouse Horticulture, Wageningen University and Research, Wageningen, Netherlands.
³ Horticulture and Product Physiology, Wageningen University and Research, Wageningen, Netherlands.

Abstract

This study analyzed interactions among photon flux density (PPFD), air temperature, root-zone temperature for growth of lettuce with non-limiting water, nutrient, and CO₂ concentration. We measured growth parameters in 48 combinations of a PPFD of 200, 400, and 750 μmol m^-2 s^-1 (16 h daylength), with air and root-zone temperatures of 20, 24, 28, and 32°C. Lettuce (Lactuca sativa cv. Batavia Othilie) was grown for four cycles (29 days after transplanting). Eight combinations with low root-zone (20 and 24°C), high air temperature (28 and 32°C) and high PPFD (400 and 750 μmol m^-2 s^-1) resulted in an excessive incidence of tip-burn and were not included in further analysis. Dry mass increased with increasing photon flux to a PPFD of 750 μmol m^-2 s^-1. The photon conversion efficiency (both dry and fresh weight) decreased with increasing photon flux: 29, 27, and 21 g FW shoot and 1.01, 0.87, and 0.76 g DW shoot per mol incident light at 200, 400, and 750 μmol m^-2 s^-1, respectively, averaged over all temperature combinations, following a concurrent decrease in specific leaf area (SLA). The highest efficiency was achieved at 200 μmol m^-2 s^-1, 24°C air temperature and 28°C root-zone temperature: 44 g FW and 1.23 g DW per mol incident light. The effect of air temperature on fresh yield was linked to all leaf expansion processes. SLA, shoot mass allocation and water content of leaves showed the same trend for air temperature with a maximum around 24°C. The effect of root temperature was less prominent with an optimum around 28°C in nearly all conditions. With this combination of temperatures, market size (fresh weight shoot = 250 g) was achieved in 26, 20, and 18 days, at 200, 400, and 750 μmol m^-2 s^-1, respectively, with a corresponding shoot dry matter content of 2.6, 3.8, and 4.2%. In conclusion, three factors determine the "optimal" PPFD: capital and operational costs of light intensity vs the value of reducing cropping time, and the market value of higher dry matter contents.

Keywords: climate management; dry matter allocation; efficiency; leaf expansion; light use efficiency; production climate; resource use efficiency; vertical farm.