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Published Erratum
. 2021 Mar 3;29(3):1349-1351.
doi: 10.1016/j.ymthe.2021.01.025. Epub 2021 Feb 15.

Immunological Synapse Predicts Effectiveness of Chimeric Antigen Receptor Cells

Wei Xiong, Yuhui Chen, Xi Kang, Zhiying Chen, Peilin Zheng, Yi-Hsin Hsu, Joon Hee Jang, Lidong Qin, Hao Liu, Gianpietro Dotti, Dongfang Liu
Published Erratum

Immunological Synapse Predicts Effectiveness of Chimeric Antigen Receptor Cells

Wei Xiong et al. Mol Ther. .
No abstract available

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Figures

Figure 2
Figure 2
(A) Confocal microscopy of Kappa-4-1BB-CAR T cells and Kappa-CD28-CAR T cells on a lipid bilayer carrying human Kappa IgG1-Alexa Fluor 647 (red). Fixed and permeabilized CAR T cells were stained for Perforin(green), pZeta (cyan), and F-actin (magenta). A visual cartoon of the lipid bilayer and CAR T cells is displayed (left). Scale bars represent 25 μm.
Figure 3
Figure 3
(A) Cytotoxicity of Kappa-CAR T cells (left) and CD19-CAR T cells (right) from the same healthy donor was measured using a standard 4-hr 51Cr-release assay. Daudi cells (the Kappa-positive B cell lymphoma cell line) were used as the Kappa-CAR T cell’s target cells. Raji cells (the CD19-positive B cell lymphoma cell line) were used as the CD19-CAR T cell’s target cells. PBMCs from a healthy donor were transduced with 4-1BB construct (red dots) or CD28 construct (black dots) retrovirus. TM (black triangle) was used as control.
Figure 4
Figure 4
(B and C) Anti-tumor effects of Kappa-CAR (B) and CD19-CAR (C) were measured by the decrease in tumor cell number (left) and increase in effector cell number (right). A Kappa-positive Daudi cell expressing fluorescent protein mCherry was used as a target cell. Error bars show ± SD. TM (black triangle) was used as control.
Figure S7
Figure S7
(A) Kappa-CAR T cells were isolated from four different individuals and transduced with TM, 4-1BB, and CD28 constructs. The target Daudi cells expressing fluorescent protein mCherry were mixed with CAR T cells for 7 days. The number of both target cells and CAR T cells were measured by flow cytometry, as described in Figure 4. (B) CD19-CAR T cells were isolated from four different individuals and transduced with TM, 4-1BB, and CD28 constructs. The Raji-GFP target cells were mixed with CAR T cells for 7 days. The number of both target cells and CAR T cells was measured by flow cytometry. These data are pooled from at least three independent experiments. TM (black triangle) was used as control.TM (black triangle) was used as control.
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