Ferroptosis-related gene signature as a prognostic marker for lower-grade gliomas

J Cell Mol Med. 2021 Mar;25(6):3080-3090. doi: 10.1111/jcmm.16368. Epub 2021 Feb 16.


Ferroptosis is a newly discovered form of programmed cell death, which has unique biological effects on metabolism and redox biology. In this study, the prognostic value of ferroptosis-related genes was investigated in lower-grade gliomas (LGG). We downloaded the ferroptosis-related genes from the FerrDb dataset. Univariate Cox and LASSO regression analyses were applied to identify genes correlated with overall survival (OS). Subsequently, 12 ferroptosis-related genes were screened to establish the prognostic signature using stepwise multivariate Cox regression. According to the median value of risk scores, patients were divided into low- and high-risk subgroups. The Kaplan-Meier curves showed the high-risk group had a lower OS. The predictive power of the risk model was validated using the CGGA. Functional analysis revealed that the terms associated with plasma membrane receptor complex, immune response and glutamate metabolic process were primarily related to the risk model. Moreover, we established a nomogram that had a strong forecasting ability for the 1-, 3- and 5-year OS. In addition, we compared the risk scores between different clinical features. We also detected infiltration of macrophages and monocytes in different subgroups. Overall, our study identified the prognostic signature of 12 ferroptosis-related genes, which has the potential to predict the prognosis of LGG.

Keywords: ferroptosis; lower-grade gliomas; prognosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Biomarkers, Tumor*
  • Brain Neoplasms / genetics*
  • Brain Neoplasms / mortality
  • Brain Neoplasms / pathology
  • Computational Biology / methods
  • Databases, Nucleic Acid
  • Ferroptosis / genetics*
  • Gene Expression Profiling*
  • Gene Expression Regulation, Neoplastic*
  • Glioma / genetics*
  • Glioma / mortality
  • Glioma / pathology
  • Humans
  • Nomograms
  • ROC Curve
  • Reproducibility of Results
  • Transcriptome*


  • Biomarkers, Tumor