MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in 3T3-L1 Cells

J Agric Food Chem. 2021 Mar 3;69(8):2564-2575. doi: 10.1021/acs.jafc.0c07451. Epub 2021 Feb 18.


The number and distribution of adipocytes directly affect the quality of livestock meat products. The analysis of the adipogenesis mechanism is the basis for improving meat quality. The formation of adipocytes is regulated by many factors, including a class of endogenous small RNAs, named microRNA (miRNA). Previous studies have shown that miRNAs could affect adipogenesis by post-transcriptional regulation of target genes. In our study, a decreased miR-99b-5p expression level was found in the adipose tissue of obese mice. Overexpression of miR-99b-5p could increase cell proliferation by promoting the cell cycle while inhibiting cell differentiation. In addition, interference with miR-99b-5p obtained the opposite result. Furthermore, the proteomics sequencing analysis screened 1154 differentially expressed proteins, which are closely related to adipocyte differentiation and fatty acid metabolism. In addition, the results of the dual-luciferase test showed that miR-99b-5p can directly target the proteins SCD1 and Lpin1 with significantly different expression levels in proteomic sequencing. Then, this result was verified at the level of mRNA and protein in a further study. Collectively, these results suggested that miR-99b-5p may be a target for improving meat quality.

Keywords: 3T3-L1 cells; Lpin1; SCD1; differentiation; miR-99b-5p; proliferation.

MeSH terms

  • 3T3-L1 Cells
  • Adipocytes
  • Adipogenesis* / genetics
  • Animals
  • Cell Differentiation
  • Mice
  • MicroRNAs* / genetics
  • Phosphatidate Phosphatase
  • Proteomics
  • Stearoyl-CoA Desaturase


  • MicroRNAs
  • Mirn99 microRNA, mouse
  • Scd1 protein, mouse
  • Stearoyl-CoA Desaturase
  • Lpin1 protein, mouse
  • Phosphatidate Phosphatase